张欣,李洺,杨嫣华,韩坤,孟存英.血清TNF-α、IL-35在炎症性肠病患者中的水平变化及其临床意义[J].内科急危重症杂志,2017,23(5):
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| 中文关键词: 肿瘤坏死因子-α 白细胞介素-35 炎症性肠病 临床意义 |
| 英文关键词:Tumor necrosis factor-α,Interleukin - 35,Inflammatory bowel disease, Clinical significance |
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| 中文摘要: |
| 【】目的：探讨血清肿瘤坏死因子-α(Tumour necrosis factor,TNF-α)、白细胞介素-35(Interleukin - 35,IL-35)在炎症性肠病(inflammatory bowel disease,IBD)患者中的水平变化及其临床意义。方法：选取2010年1月至2016年1月期间本院消化内科住院部IBD患者120例,其中60例克罗恩病(Crohn’s disease,CD)患者作为CD组,60例溃疡性结肠炎(ulcerative colitis,UC)患者作为UC组,同期选取健康体检中心的60例健康志愿者作为对照组,采用双抗夹心酶联免疫吸附试验(Double sandwich enzyme-linked immunosorbent assay,DSA-ELISA)检测血清TNF-α、IL-35水平。结果：CD组与UC组患者血清TNF-α水平明显高于对照组,血清IL-35水平明显低于对照组,P<0.05。随着CD、UC患者病情活动度增加,血清TNF-α水平逐渐增加,血清IL-35水平逐渐降低,不同病情活动度血清TNF-α、IL-35水平比较差异具有统计学意义(P<0.05)。经Pearson直线相关性分析显示,CD组患者血清TNF-α水平与血清IL-35水平呈负相关(r=-0.412,P<0.001)；UC组患者患者血清TNF-α水平与血清IL-35水平呈负相关(r=-0.423, P<0.001)。结论：炎症性肠病患者血清TNF-α水平明显升高,血清IL-35水平明显降低,且改变幅度与病情活动度具有紧密的关系。 |
| 英文摘要: |
| Objective:To study the changes and its clinical significance of serum Tumour necrosis factor-α(TNF-α), Interleukin - 35(IL-35) levels in inflammatory bowel disease(IBD).Methods: 120 cases patients with IBD from Digestive System in-patient Department of our hospital during the period of January 2010 and January 2016 were selected,which 60 cases patients with Crohn’s disease(CD) were as CD group, 60 cases patients with ulcerative colitis(UC) were as UC group, at the same time,60 cases of healthy volunteers from healthy physical examination center were as control group, serum TNF-α, IL - 35 levels were detected by Double sandwich enzyme - linked immunosorbent assay(DSA - ELISA).Results: The serum TNF-α levels of CD group and UC group patients were significantly higher than the control group, the serum TNF-α, IL - 35 levels were significantly lower than the control group, P < 0.05.With the increase of disease activity in CD and UC patients, serum TNF-α levels were increased gradually, serum IL - 35 levels were decreased gradually , serum TNF-α, IL - 35 levels compared among different disease activity with statistically significant differences (P < 0.05).The straight Pearson correlation analysis showed, serum TNF-α levels of CD patients were negatively correlated to serum IL - 35 levels(r =-0.412, P < 0.001),serum TNF-α levels of UC patients were negatively correlated to serum IL - 35 levels(r =-0.423, P < 0.001). Conclusion: The serum TNF-α is significantly increased and serum IL - 35 is significantly decreased in inflammatory bowel disease , and the changes has a significantly close relationship with disease activity. |
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