• 吡非尼酮抑制转化生长因子β1诱导肺成纤维细胞胶原合成的实验研究
  • 〗Inhibitory effect of pifenidone on collagen synthesis in lung fibroblasts induced by TGF-β1
  • 周俊平,陈德文,郑艳,黄贞.吡非尼酮抑制转化生长因子β1诱导肺成纤维细胞胶原合成的实验研究[J].内科急危重症杂志,2019,25(5):410-412
    DOI:10.11768/nkjwzzzz20190517
    中文关键词:  吡非尼酮  肺成纤维细胞  p38MAPK  胶原  TGF-β1
    英文关键词:Pirfnidone  Lung fibroblasts  p38MAPK  Collagen  TGF-β1
    基金项目:
    作者单位
    周俊平 枣庄矿业集团中心医院 
    陈德文  
    郑艳  
    黄贞  
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    中文摘要:
          目的: 探讨吡非尼酮对转化生长因子β1(TGF-β1)诱导的肺成纤维细胞胶原合成的影响及机制。方法 : 以人胚胎肺成纤维MRC-5细胞为研究对象,用p38丝裂原活化蛋白激酶(p38MAPK)信号抑制剂SB203580和吡非尼酮处理TGF-β1诱导的肺成纤维细胞,MTT检测细胞增殖, Western blot检测细胞中Ⅲ型胶原酶(Col Ⅲ)、I型胶原酶(ColⅠ)蛋白表达和p38MAPK磷酸化水平。结果: TGF-β1诱导处理后的肺成纤维细胞增殖能力升高,细胞中Col Ⅲ、ColⅠ和p38MAPK磷酸化水平升高。吡非尼酮处理可以降低TGF-β1诱导的肺成纤维细胞增殖能力,减少细胞中Col Ⅲ、ColⅠ和p38MAPK磷酸化蛋白表达。p38MAPK信号抑制剂SB203580处理具有协同吡非尼酮降低TGF-β1诱导的肺成纤维细胞增殖和表达蛋白Col Ⅲ、ColⅠ及p38MAPK磷酸化水平的作用。结论: 吡非尼酮通过抑制p38MAPK信号,从而抑制TGF-β1诱导的肺成纤维细胞胶原合成。
    英文摘要:
          Objective: To investigate the effect of pirfnidone on collagen synthesis induced by TGF-β 1 in lung fibroblasts and its mechanism. Methods: Human embryonic lung fibroblast MRC-5 cells were used as objects of study. TGF-β 1 induced collagen synthesis in lung fibroblasts. The cells were treated with pirfnidone, cell proliferation was measured by MTT, Western blotting was used to detect the expression of Col III and Col I protein, and the level of p38MAPK phosphorylation in cells. The p38MAPK signal inhibitor SB203580 and pirfnidone were used to treat TGF-β1-induced lung fibroblasts. MTT was used to detect cell proliferation, Western blotting was used to detect the expression of Col III and Col I protein, and the level of p38MAPK phosphorylation in cells. Results TGF-β 1 induced increased proliferation of lung fibroblasts after treatment, and the phosphorylation level of Col III, Col I and p38MAPK increased in cells. Pirfidone treatment could reduce the proliferation of lung fibroblasts induced by TGF-β 1, and inhibit the expression of Col III, Col I and p38MAPK phosphorylation proteins in cells. The treatment of SB203580 could cooperate with pirfenidone to reduce the proliferation of lung fibroblasts induced by TGF-β1 and the phosphorylation levels of Col III, Col I and p38 MAPK. Conclusion: Pirfnidone inhibits TGF-β1-induced collagen synthesis in lung fibroblasts by inhibiting the p38MAPK signaling.