吡非尼酮抑制转化生长因子β1诱导肺成纤维细胞胶原合成的实验研究
Protective effect of Xuebijing injection on liver injury in severe acute pancreatitis and its effect on the expression of hepatic signal transduction and activator of transcription (p-STAT3)

周俊平.吡非尼酮抑制转化生长因子β1诱导肺成纤维细胞胶原合成的实验研究[J].内科急危重症杂志,2019,25(5):410-412

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DOI：10.11768/nkjwzzzz20190517

英文关键词:Severe acute pancreatitis Acute liver injury Xuebijing P-STAT3 signaling pathway

基金项目:基金项目：湖北省教育厅科研项目 (No：B2016116)

作者	单位	E-mail
周俊平	枣庄矿业集团中心医院	2738920058@qq.com

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中文摘要:

目的：探讨吡非尼酮对转化生长因子β1（TGF-β1）诱导的肺成纤维细胞胶原合成的影响及机制。方法：以人胚胎肺成纤维MRC-5细胞为研究对象，用p38丝裂原活化蛋白激酶（p38MAPK）信号抑制剂SB203580和吡非尼酮处理TGF-β1诱导的肺成纤维细胞，MTT检测细胞增殖， Western blot检测细胞中Ⅲ型胶原酶（Col Ⅲ）、I型胶原酶（ColⅠ）蛋白表达和p38MAPK磷酸化水平。结果： TGF-β1诱导处理后的肺成纤维细胞增殖能力升高，细胞中Col Ⅲ、ColⅠ和p38MAPK磷酸化水平升高。吡非尼酮处理可以降低TGF-β1诱导的肺成纤维细胞增殖能力，减少细胞中Col Ⅲ、ColⅠ和p38MAPK磷酸化蛋白表达。p38MAPK信号抑制剂SB203580处理具有协同吡非尼酮降低TGF-β1诱导的肺成纤维细胞增殖和表达蛋白Col Ⅲ、ColⅠ及p38MAPK磷酸化水平的作用。结论：吡非尼酮通过抑制p38MAPK信号，从而抑制TGF-β1诱导的肺成纤维细胞胶原合成。

英文摘要:

Objective: To investigate the effect of Xuebijing on severe acute pancreatitis (SAP) -associated acute liver injury in the rats and explore the underlying mechanisms. Methods: Male Sprague-Dawley (SD) rats (n=60) were randomly divided into sham-operated group (n=20), SAP model group (n=20), and treatment group (n=20). SAP model was induced by retrograde injection of 5% sodium taurocholate (1 mL/kg) into the biliopancreatic duct. The rats in Xuebijing treatment group were intraperitoneally injected with 4 mL/kg Xuebijing. The rats were sacrificed at 12 h (n=10) and 24 h (n=10) after the treatment. The levels of serum ALT and AST were determined by the puncture through inferior vena cava. The serum levels of TNF-α and IL-6 were examined by ELISA, and the protein levels of p-STAT3 in the liver were evaluated by Western blotting. Results: As compared with sham-operated group，the levels of serum ALT, AST, IL-6 and TNF-α in SAP model group were significantly increased, and those in Xuebijing treatment group were significantly decreased as compared with those in model group. Meanwhile, the phosphorylation levels of p-STAT3 were significantly increased in model group as compared with sham-operated group. The rats in Xuebijing treatment group had lower phosphorylation levels of STAT3. Conclusion: Xuebijing exerts a protective effect on pancreatitis-associated acute liver injury in the rats possibly via inhibiting p-STAT3 signaling pathway.