血必净对重症急性胰腺炎肝损伤的保护作用
Protective effect of Xuebijing injection on liver injury in severe acute pancreatitis and its effect on the expression of hepatic signal transduction and activator of transcription (p-STAT3)

王晓君.血必净对重症急性胰腺炎肝损伤的保护作用[J].内科急危重症杂志,2019,25(5):418-420

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DOI：10.11768/nkjwzzzz20190519

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基金项目:湖北省教育厅科研项目 (No：B2016116)

作者	单位	E-mail
王晓君	湖北省十堰市太和医院（湖北医药学院附属医院）	liang63521@163.com

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中文摘要:

目的：研究血必净对重症急性胰腺炎(SAP)大鼠肝损伤的保护作用及其对肝脏信号传导与转录激活因子3(p-STAT3)表达的影响。方法：60只6～8周龄清洁级雄性SD大鼠按照随机数字表法分成3组：胰腺炎模型组、假手术组、血必净组,每组20只。胰腺炎模型组和血必净组采用5%牛磺胆酸钠逆行胰胆管注射制备重症SAP大鼠模型，假手术组打开腹腔后不注射药物，血必净组在造模后腹腔内1次性注射血必净4mL/kg。造模成功后12、24h,3组随机各取大鼠10只，心脏穿刺取血5mL，应用全自动生化分析仪检测血清丙氨酸转氨酶（ALT）、天门冬氨酸转氨酶（AST），ELISA法检测血清IL-6、TNF-α水平，Western blotting法检测肝脏p-STAT3蛋白表达，取血后处死，取一叶肝组织制作石蜡切片，进行HE染色和免疫组化染色,观察肝组织损伤程度及p-STAT3蛋白表达情况。结果：胰腺炎模型组和血必净组大鼠ALT、AST水平显著升高，炎性因子IL-6、TNF-α及p-STAT3蛋白表达较假手术组均明显升高（均P<0.05），血必净组转氨酶ALT、AST水平、IL-6、TNF-α、p-STAT3蛋白表达水平均较胰腺炎模型组低（均P<0.05）。结论：血必净对大鼠SAP肝损伤有保护作用，可能与调控p-STAT3信号通路，抑制促炎细胞因子过度表达有关。

英文摘要:

Objective: To investigate the effect of Xuebijing on severe acute pancreatitis (SAP) -associated acute liver injury in the rats and explore the underlying mechanisms. Methods: Male Sprague-Dawley (SD) rats (n=60) were randomly divided into sham-operated group (n=20), SAP model group (n=20), and treatment group (n=20). SAP model was induced by retrograde injection of 5% sodium taurocholate (1mL/kg) into the biliopancreatic duct. The rats in Xuebijing treatment group were intraperitoneally injected with 4mL/kg Xuebijing. The rats were sacrificed at 12h (n=10) and 24h (n=10) after the treatment. The levels of serum ALT and AST were determined by the puncture through inferior vena cava. The serum levels of TNF-α and IL-6 were examined by ELISA, and the protein levels of p-STAT3 in the liver were evaluated by Western blotting. Results: As compared with sham-operated group，the levels of serum ALT, AST, IL-6 and TNF-α in SAP model group were significantly increased, and those in Xuebijing treatment group were significantly decreased as compared with those in model group. Meanwhile, the phosphorylation levels of p-STAT3 were significantly increased in model group as compared with sham-operated group. The rats in Xuebijing treatment group had lower phosphorylation levels of STAT3. Conclusion: Xuebijing exerts a protective effect on pancreatitis-associated acute liver injury in the rats possibly via inhibiting p-STAT3 signaling pathway.