• 血浆miRNA-190 及miRNA-197联合检测可辅助诊断肺血栓栓塞症
  • Combined detection of miRNA-190 and miRNA-197 is conducive to the diagnosis of pulmonary thromboembolism
  • 周晓婷.血浆miRNA-190 及miRNA-197联合检测可辅助诊断肺血栓栓塞症[J].内科急危重症杂志,2021,27(5):397-400
    DOI:10.11768/nkjwzzzz20210510
    中文关键词:  肺血栓栓塞  血浆miRNA-190  血浆miRNA-197  联合诊断
    英文关键词:
    基金项目:苏州市第九人民医院科研基金项目(院201726、YK202109)
    作者单位E-mail
    周晓婷 苏州市第九人民医院呼吸与危重症医学科 cx_shishi@163.com 
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    中文摘要:
          目的:探讨血浆miRNA-190和miRNA-197在肺血栓栓塞症(PTE)患者中表达水平及辅助诊断价值。方法:选择2017年7月至2019年6月苏州市第九人民医院PTE患者30例为PTE组,同期匹配心肌梗死患者45例为心肌梗死组,健康体检者45例为正常对照组。采用实时荧光定量PCR(qRT-PCR)检测血浆miRNA-190和miRNA-197在所有受试者外周血中的表达水平,并分析其在3组中的表达量差异。应用受试者工作特征曲线(ROC曲线)及曲线下面积(AUC)(95% CI )评估两者单独或联合检测作为PTE诊断指标的意义。结果:血浆miRNA-190在3组中的相对表达量分别为3.01±2.01,1.80±1.02,1.18±0.67;其在PTE组血浆中表达量较心肌梗死组及正常对照组高( t =3.602, t = 5.814, P 均<0.05)。血浆miRNA-197在3组中相对表达量分别为4.82±3.80,1.88±1.50, 1.38±0.83;其在PTE组血浆中表达量较心肌梗死组及正常对照组高( t =4.791, t =5.886, P 均< 0.05)。作为PTE的诊断指标,血浆miRNA-190的最佳临界值为1.598,敏感度为75.56%,特异度为80.00%,AUC为0.7844,95% CI 为0.6858~0.8831;血浆miRNA-197最佳临界值为2.40,敏感度为75.56%,特异度为 86.67%,AUC为0.7931,95% CI 为0.6870~0.8991。血浆miRNA190和miRNA-197联合检测诊断PTE的敏感度为80.00%,特异度为93.33%,AUC为0.9136,95% CI 为 0.8516~0.9756;均高于两者单独检测。结论:血浆miRNA-190和miRNA-197在PTE患者外周血血浆中表达量明显升高,两者联合检测敏感度和特异度更高,有助于PTE的辅助诊断。
    英文摘要:
          Objective: To study the diagnostic value of quantitative detection of plasma miRNA-190 and miRNA-197 in patients with pulmonary thromboembolism (PTE). Methods: Totally, 30 patients diagnosed with PTE admitted to Respiratory Medicine of Suzhou Ninth People's Hospital from July 2017 to June 2019 were enrolled in this study and plasma was collected. A total of 45 patients with myocardial infarction (MCI) and 45 healthy participants were recruited as the control group. qRT-PCR was used to detect the relative expression level of miRNA-190 and miRNA-197. The diagnostic value was evaluated by receiver operating characteristic (ROC) curves and the area under the ROC curve (AUC) (95%CI). Results: The plasma level of miRNA-190 in PTE group, MCI group and healthy group was 3.01±2.01, 1.80±1.02 and 1.18±0.67 respectively. The plasma level of miRNA-190 in PTE group was significantly higher than in MCI group and healthy group (t=3.602, t=5.814, P all< 0.0001). The plasma level of miRNA-197 in PTE group, MCI group and healthy group was 4.82±3.80, 1.88±1.50, 1.38±0.83 respectively, and the relative expression of miRNA-197 in PTE group was significantly higher than that in the other two groups (t=4.791, t=5.886, P all<0.05). As the diagnostic indicator, the sensitivity and specificity of miRNA-190 were 75.56% and 80% respectively, with the AUC being 0.7844 (95%CI: 0.6858-0.8831). The sensitivity and specificity of miRNA-197 was 75.56% and 86.67% and the AUC was 0.7931 (95%CI: 0.6870-0.8991). However, the combined detection of miRNA-190 and miRNA-197 showed the sensitivity and specificity of 80.00% and 93.33% respectively, and the AUC was 0.9136 (95%CI: 0.8516-0.9756). Conclusion: The relative expressions of miRNA-190 and miRNA-197 in PTE patients were significantly higher than those in MCI group and healthy group. Combined detection of miRNA-190 and miRNA-197 shows higher sensitivity and specificity, which is more conducive to the diagnosis of PTE.