李洁.高通量单细胞及空间转录组技术在肝脏疾病中的应用[J].内科急危重症杂志,2023,29(3):177-180
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| DOI：10.11768/nkjwzzzz20230301 |
| 中文关键词: 肝脏 感染 单细胞转录组技术 空间转录组 |
| 英文关键词: |
| 基金项目:国家自然科学基金面上项目（No:81703149）；湖南省杰青（No:2023JJ10095）；湖南省创新人才工程（No:2022RC1212） |
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| 中文摘要: |
| 肝脏是人体重要的免疫器官，在检测、捕获、清除病原体和外来抗原中发挥着重大作用。本文主要简述高通量单细胞及空间组学技术解析肝脏在感染性疾病中的应用。随着高通量单细胞技术的发展，现在可以在单细胞水平上破译复杂器官和疑难疾病的异质性、分化、细胞间通信等，从而认识到过去未知的致病途径和疾病机制，有利于开发新的治疗靶点。并且随着技术不断成熟，单细胞转录组可以和空间转录组学、表观遗传组、蛋白组、代谢组、微生物组以及临床数据进行整合分析，有益于将患者分层并进行精准治疗。 |
| 英文摘要: |
| The mammalian liver is a front-line immune organ that plays a vital role in the detection, capture and clearance of pathogens and foreign antigens entering the human body. With advancement of burgeoning high-throughput single-cell technology, we can now decipher the heterogeneity, differentiation, and intercellular crosstalk at single cell level, in sophisticated organs and diseases. In this concise review, we summarize how emerging high-throughput single-cell technologies have advanced or re-defined our understanding of the liver function toward infection, and unravel unknown pathogenic pathways and disease mechanisms for the development of new therapeutics. Moreover, as these technologies mature, their integration into spatial transcriptomics, epigeneticomics, proteomics, metabolomics, microbiome and clinical data analysis will aid in patient stratification and in developing treatment plans for patients suffering from infectious disease. |
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