• 流式细胞术检测皮肤组织在母细胞性浆细胞样树突细胞肿瘤诊断中的应用
  • 郑凤丽.流式细胞术检测皮肤组织在母细胞性浆细胞样树突细胞肿瘤诊断中的应用[J].内科急危重症杂志,2024,30(3):224-228
    扫码阅读全文 本文二维码信息
    DOI:10.11768/nkjwzzzz20240306
    中文关键词:  流式细胞术  皮肤;母细胞性浆细胞样树突细胞肿瘤
    英文关键词:
    基金项目:
    作者单位
    郑凤丽 华中科技大学同济医学院附属同济医院 
    摘要点击次数: 103
    全文下载次数: 144
    中文摘要:
          摘要 目的:探讨流式细胞术(FCM)检测皮肤组织样本在诊断母细胞性浆细胞样树突细胞肿瘤(BPDCN)中的作用。方法:利用多色流式检测皮肤组织与骨髓,并结合组织病理学、骨髓细胞形态学、遗传学、二代测序(NGS)等检测结果对3例已确诊的BPDCN进行回顾性分析。结果:3例患者均以皮肤损害为首发症状,FCM检测骨髓仅发现1例患者标本存在BPDCN细胞,而3例患者皮肤组织标本中均发现BPDCN细胞;与皮肤组织病理免疫组化结果诊断一致;病例2患者骨髓细胞学和遗传学检查均未发现BPDCN细胞,病例1患者骨髓细胞学中发现BPDCN细胞,病例3患者NGS检测到BPDCN相关基因突变。结论:流式细胞免疫分型是BPDCN诊断的实用辅助手段,可以在早期皮肤组织标本中快速精准地诊断BPDCN,同时进行鉴别诊断。
    英文摘要:
          Abstract Objective: To investigate the value of flow cytometry (FCM) in the diagnosis of blastic plasmacytoid dendritic cell neoplasm (BPDCN) by detecting cutaneous tissue samples. Methods: The cutaneous tissue and bone marrow were detected by multi-color FCM, and the results of histopathology, bone marrow cell morphology, genetics, and second-generation sequencing were analyzed retrospectively in 3 cases of confirmed BPDCN. Results: All 3 patients had skin damage as the initial symptom. FCM detection of bone marrow only found BPDCN cells in one patient sample, while FMC detection of skin tissue samples found BPDCN cells in all 3 patients. The diagnosis was consistent with the pathological and immunohistochemical diagnosis of skin tissue. The bone marrow cytology and genetic examination in one patient (case 2) did not detect BPDCN cells. BPDCN cells were found in bone marrow cytology of one patient (case 1), and BPDCN related gene mutations were detected in one patient (case 3) by second-generation sequencing. Conclusion: FCM immunophenotyping is a practical auxiliary tool for the diagnosis of BPDCN, which can quickly and accurately diagnose BPDCN in early skin tissue specimens, and perform differential diagnosis. It is an effective pathological auxiliary tool.