• 支链氨基酸有效预测慢性阻塞性肺病的急性加重
  • 武小杰.支链氨基酸有效预测慢性阻塞性肺病的急性加重[J].内科急危重症杂志,2024,30(3):243-246
    扫码阅读全文 本文二维码信息
    DOI:10.11768/nkjwzzzz20240310
    中文关键词:  慢性阻塞性肺病  支链氨基酸  细胞因子  炎症机制
    英文关键词:
    基金项目:武汉市卫健委科研项目(WX21Q07)
    作者单位
    武小杰 武汉市第一医院 
    摘要点击次数: 91
    全文下载次数: 151
    中文摘要:
          摘要 目的:探讨支链氨基酸(BCAAs)代谢在慢性阻塞性肺病(COPD)发生机制中的作用。方法:收集42例COPD稳定期患者纳入COPD组,40例COPD急性加重(AECOPD)期患者纳入AECOPD组,收集同期46例健康体检者为对照组,采集外周血,应用液相色谱-质谱分析检测BCAAs水平,酶联免疫吸附(ELISA)法检测炎症因子水平,并对二者的相关性进行分析。结果:与对照组比较,COPD组及AECOPD组患者血浆亮氨酸、缬氨酸水平更低,且AECOPD组低于COPD组;血浆白介素(IL)-8、C-反应蛋白(CRP)水平更高,且AECOPD组高于COPD组(P均<0.001)。血浆中亮氨酸、缬氨酸水平与IL-8、CRP水平呈负相关(r=-0.64、-0.71、-0.73、-0.73,P均<0.05)。结论:BCAAs代谢可能通过炎症反应参与COPD的发生,临床中BCAAs水平有可能成为检测COPD急性加重潜在的生物标志物。
    英文摘要:
          Abstract Objective: To investigate the role of branched-chain amino acids (BCAAs) metabolism in the pathogenesis of chronic obstructive pulmonary disease (COPD). Methods: Totally, 42 patients with stable COPD were included in the COPD group, 40 patients with acute exacerbation of COPD served as the AECOPD group, and 46 healthy subjects were fallen into the control group. BCAAs levels in peripheral blood were detected by liquid chromatography tandem mass spectrometry and the levels of cytokines in peripheral blood were detected by enzyme-linked immunosorbent assay (ELISA). The correlation between them was analyzed. Results: Compared with the control group, the serum leucine and valine levels in COPD group and AECOPD group were lower, and those in the AECOPD group were lower than those in the COPD group. Compared with the control group, the plasma levels of IL-8 and CRP in the COPD group and AECOPD group were increased, and those in the AECOPD group were higher than those in the COPD group (all P< 0.001). Plasma leucine and valine levels were negatively correlated with IL-8 and CRP levels (r=-0.64, -0.71, -0.73, -0.73, P< 0.05). Conclusion: BCAAs may be involved in the development of COPD through inflammatory response, and BCAAs may be potential biomarkers for detecting AECOPD.