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| DOI： |
| 中文关键词: 慢性阻塞性肺病 支链氨基酸 细胞因子 炎症机制 |
| 英文关键词:?Chronic obstructive pulmonary disease Branched chain amino acid cytokines Cytokines Inflammatory pathogenesis |
| 基金项目:武汉市卫健委科研项目( No:WX21Q07) |
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| 中文摘要: |
| 目的：分析支链氨基酸（BCAAs）在慢性阻塞性肺病（COPD）稳定期与急性加重期患者外周血中的水平，结合炎症指标探讨氨基酸代谢在COPD发生机制中的作用。方法：收集42例COPD稳定期患者纳入COPD组，40例COPD急性加重期患者纳入AECOPD组，收集同期46例健康体检者作为对照组，采集外周血，应用液相色谱-质谱分析检测BCAAs水平，ELISA方法检测炎症因子水平，并对两者的相关性进行分析。结果：与对照组相比，COPD组及AECOPD组患者血亮氨酸、缬氨酸水平降低，血IL-8、CRP水平升高，差异有统计意义（P＜0.05）。与COPD组相比，AECOPD组BCAAs水平下降程度更高，血IL-8、CRP水平升高程度更高，差异有统计意义（P＜0.05）。亮氨酸、缬氨酸水平与IL-8,CRP水平呈负相关，差异有统计意义（P＜0.05）。结论：BCAAs代谢可能通过炎症反应参与COPD的发生，临床中BCAAs水平有可能成为检测COPD急性加重潜在的生物标志物。 |
| 英文摘要: |
| Objective To analyze the levels of branched-chain amino acids (BCAAs) in peripheral blood of patients with chronic obstructive pulmonary disease (COPD) in stable and acute exacerbations, and to explore the role of amino acid metabolism in the pathogenesis of COPD combined with inflammatory indicators.Methods Forty-two patients with stable COPD were included in the COPD group, forty patients with acute exacerbation of COPD were included in the AECOPD group, and forty-six healthy subjects were included in the control group. BCAAs levels in peripheral blood were detected by LC-MS and the levels of cytokines in peripheral blood were detected by ELISA.Results Compared with the control group, the serum leucine and valine levels in COPD group and AECOPD group were decreased, and the serum IL-8 and CRP levels were increased (P < 0.05). Compared with COPD group, the level of BCAAs in AECOPD group was lower, and the levels of IL-8 and CRP in blood was higher (P < 0.05).Conclusion BCAAs may be involved in the development of COPD through inflammatory response,and BCAAs may be a potential biomarker for detecting acute exacerbation of COPD. |
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