肺癌组织中Yes相关蛋白的表达与临床特征相关

邓科兰.肺癌组织中Yes相关蛋白的表达与临床特征相关[J].内科急危重症杂志,2021,27(3):234-237

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DOI：10.11768/nkjwzzzz20210314

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基金项目:湖北省自然科学基金项目（No:2014CFC1059）

作者	单位	E-mail
邓科兰	武汉科技大学附属孝感医院呼吸与危重症医学科	qingzhengp@tom.com

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中文摘要:

目的：探讨肺癌组织中转录共激活因子蛋白（YAP）的表达与临床特征的关系及对预后的预测价值。方法：选取孝感市中心医院2015年4月-2016年12月收治的50例手术切除新鲜肺癌标本和癌旁组织标本及同期50例肺部良性病变患者经皮肺穿刺活检获得的正常肺组织标本，采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连结（SP）法和Westernblot法检测标本中YAP的表达水平，分析其与肺癌临床病理特征及预后的相关性。结果：①免疫组化结果：YAP在36例肺癌组织中呈强阳性表达，在14例肺癌组织中呈阴性或弱阳性表达，在癌旁组织与正常组织中均呈阴性或弱阳性表达，差异有统计学意义（P均＜0.05）;②Westernblot结果显示，肺癌组织中YAP的相对表达量明显高于癌旁组织与正常组织，差异有统计学意义（P均＜0.05）;③在肺癌组织中YAP的表达水平与患者的性别、年龄、肿瘤类型、病灶直径、TNM分期、分化程度、胸壁转移及血清细胞角蛋白19可溶性片段(CYFRA21-1)、神经元特异性烯醇化酶(NSE)、糖类抗原125(CA125)水平无关（P均＞0.05），与淋巴结转移有关（P<0.05）;④YAP高表达组与低表达组的无复发生存率比较，差异无统计学意义（P>0.05）;⑤肺癌预后与TNM分期、淋巴结转移有关（P均<0.05），与YAP表达水平无关(P>0.05）。结论：在肺癌组织中YAP的表达上调，与肺癌生物学行为有一定关系，但在预测肺癌预后中的价值有限。

英文摘要:

Objective: To investigate the relationship between the expression of YAP in lung cancer tissues and clinical characteristics and its prognostic value. Methods: A total of 50 fresh lung cancer specimens and paracancer tissue specimens from April 2015 to December 2016 were resected surgically and 50 normal tissue samples with benign lung lesions were obtained by percutaneous lung biopsy. The expression of YAP in the samples was detected by immunohistochemical SP method and Western blotting, and its correlation with clinicopathological features and prognosis of lung cancer was analyzed. Results: ① Immunohistochemical results revealed that YAP showed strong positive expression in 36 lung cancer tissues, negative or weak positive expression in 14 lung cancer tissues, negative or weak positive expression in paracancer tissues and normal tissues, and the difference was statistically significant ( all P< 0.05). ② Western blotting results showed the relative expression of YAP in lung cancer tissues was significantly higher than that in paracancer tissues and normal tissues (P< 0.05). ③ The expression level of YAP in lung cancer tissues was not related to patients' gender, age, tumor type, diameter, TNM stage, differentiation degree, chest wall metastasis, serum cyfra21-1, NSE and CA125 levels (all P> 0.05), but to lymph node metastasis (P< 0.05). ④ There was no statistically significant difference in relapse-free survival between the high expression group of YAP and the low expression group (P> 0.05). ⑤ The prognosis of lung cancer was related to TNM stage and lymph node metastasis( all P< 0.05), while not to the expression level of YAP(P> 0.05). Conclusion: The expression of YAP in lung cancer was up-regulated, and is related to the biological behavior of lung cancer, while has limited value in predicting the prognosis of lung cancer.